Coding
Part:BBa_K3286265:Design
Designed by: Marijn Ceelen Group: iGEM19_Wageningen_UR (2019-10-21)
Phage T7 P10 without stop codon + translation initiation sequence + T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 84
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 372
Illegal BsaI.rc site found at 20
Illegal BsaI.rc site found at 576
Design Notes
Removed XbaI site at the start of the sequence in a non coding part to be biobrick compatible.
Source
Sequence amplified from the T7 genome by PCR